Installing the TREND package under Windows, OS X or Linux
To obtain access to TREND, go to http://biochem.missouri.edu/trend/
Submit either an academic license agreement or request for commercial licensing, as well as the contact information for you and your laboratory. Await approval and an email message providing download access to the TREND distributions.
Though written in Python, TREND does not depend on a Python environment. The simple installation process merely creates shortcuts on the desktop and helps set environment variables of for the text shell window that will make TREND easy to use. The installation script places on the Desktop a directory containing symbolic links to the executable files with GUI.
The OS X and Linux installation script change the PATH environment variable. 
The Windows script TRENDterminal.bat opens a command shell in which all 
executable files of TREND (with suffix of .exe) can be invoked, both the 
versions operated by GUI and at the command line. 
Note that the installation process is not completed until the first 
calculations using trendmain and trendreconstruct trigger the download 
and installation of two pieces of software from the public domain for 
handling movies and images. This download requires both a reliable internet 
connection and access to github. If this access is lacking upon initial 
usage, please see the document no_internet_connection.pdf
windows
Windows 7 or above
- Unzip the 
TREND-1.x-Win.7zby 7zip. - In the unzipped folder, double clicking 
install.batwill create a file namedTRENDterminaland a folder on the desktop and start menu namedTREND-GUIcontainingtrendmaingui.exe,trendplotgui.exe, andtrendreconstructgui.exe. Refresh the desktop by right-clicking in an open area and selectRefresh. - Invoke any of these GUI versions of the executable files by double-clicking
on them within the 
TREND-GUIfolder. If TREND was previously installed, the old version will be uninstalled before new version is installed. - An alternative way to run either the GUI or command-line versions of the
executable files involves first double clicking on 
TRENDterminal.bat. This will open a Windows command shell in which you cancdto your data folder and invoke the TREND executable files. This does not change your path environment permanently and should be run every time you need a command shell window for TREND. - Note the 
.7ztarball can be deleted but the unzipped folder should not be removed or deleted, since the installation step just creates shortcuts. 
macos
Mac OS X 10.7.5 and later
- See Known Limitations by OS if TREND apps can't be launched on OSX 10.11 and later
 - Unzip the 
TREND-1.x-macOS.tar.gzby double clicking the file displayed in the OS X GUI. Alternatively, run the commandtar -xzvf TREND-1.x-macOS.tar.gzwithin a terminal shell. - Open a terminal shell and 
cdto the unzipped folder namedTREND-0.7.8.3-OSX, enter the command./install.scriptto create aTREND-GUIfolder on the desktop, which containstrendmaingui.app,trendplotgui.app, andtrendreconstructgui.app. The installation scripts can only be executed within the terminal shell. (Do not run this script directly from OS X desktop GUI by clicking because the installation script will not work properly). - Three icons, 
trendmaingui.app,trendplotgui.app, andtrendreconstructgui.appwill also be added to/Applications/TREND. These icons should be visible in Launchpad and the Applications folder in Finder. (Refreshing Launchpad may be needed). - The current TREND directory is also added to the 
PATHenvironment variable. Three lines will be added to the file~/.bash_profile. The lines begin with#TREND path environment - Open a new terminal or type and enter the command source 
~/.bash_profilewithin a terminal shell. Now all TREND commands can be launched in a terminal. - If TREND was previously installed, the old version will be uninstalled before the new installation.
 sudomay be necessary if permission is required.
An example: DownloadTREND-1.x-macOS.tar.gzto~/Downloads/- If you are comfortable with shell terminal, you can just enter 
./change_path_env.scriptto add the current TREND directory toPATHenvironment variable. No permission is required.tar -xzvf TREND-1.x-macOS.tar.gz cd ~/Downloads/TREND-1.x-macOS ./install.script (or sudo ./install.script if permission is required) 
linux
Linux 64-bit, including Ubuntu 14.04, 16.04, Fedora 23 and later,
RedHat/CentOS 7.x (7.1 and later), CentOS 6.x (6.5 and later)
- Unzip the 
TREND-1.x-Ubuntu14.04.tar.gz(The stringUbuntu14.04can be any other Linux distribution such asRHEL7.1orUbuntu16.04) by entering the commandtar -xzvf TREND-1.x-Ubuntu14.04.tar.gzwithin a terminal shell. - Enter 
./install.scriptwithin the terminal shell to add the current TREND directory to thePATHenvironment variable. Three lines will be added to~/.bashrc, starting with#TREND path environment. - Open a new terminal or type 
source ~/.bashrc. Now all TREND commands can be launched in terminal. - Installation in Linux is similar to OS X, as the 
install.scriptis written forbash, a shell that is the default in Mac OS X and many Linux distributions. - However, a Linux computer may run without a desktop and may
use a shell other than 
bash. In this case, you need to set the path environment variables yourself. e.g., modifying a shell configuration file such as.bashrc,.cshrc,.tcshrc....
For example, if you want to install TREND on a CentOS 6.5 platform withtcshorcshenvironment, just add the following lines to your.cshrcor.tcshrcfiles:Replaceset path = ($path ~/your_directory/TREND-1.x-CentOS6.5/CLI) set path = ($path ~/your_directory/TREND-1.x-CentOS6.5/GUI)your_directorywith the paretn directory name of the TREND folder. 
Uninstalling or Moving
- Run 
uninstall.batin Windows oruninstall.scriptin OS X and Linux. - Then delete the 
TREND-1.x-Win,TREND-1.x-macOS, orTREND-1.x-Ubuntu14.04folder. - To move the TREND folder to a new location, first uninstall it, move the TREND foler to the new location, and install it again, thereby overwriting the previously installed TREND executable files.